Biotechnology
Principles, processes and applications of biotechnology including rDNA technology.
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Take a full mock →Q1 · Biotechnology · MEDIUM
A researcher is working with recombinant plasmids to clone a target gene in E. coli. If the plasmid contains an ampicillin resistance gene and a tetracycline resistance gene, and the foreign DNA is inserted into the tetracycline resistance gene, which of the following colonies will represent successful transformants?
Q2 · Biotechnology · EASY
Which of the following enzymes is essential for creating recombinant DNA by joining DNA fragments from different sources?
Q3 · Biotechnology · MEDIUM
In the polymerase chain reaction (PCR), if a DNA sample initially contains 8 copies of the target sequence and undergoes 5 complete cycles, how many copies of the target sequence will be present?
Q4 · Biotechnology · HARD
In gene therapy for adenosine deaminase (ADA) deficiency, lymphocytes are extracted from the patient, genetically modified, and reintroduced. Which vector system is most commonly employed for delivering the functional ADA gene into these cells?
Q5 · Biotechnology · MEDIUM
In recombinant DNA technology, restriction endonucleases are essential tools. Which characteristic is most important for a restriction enzyme to be useful in gene cloning?